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2021 OMIG Abstract
Sjögren’s Syndrome and Composition of the Gut Microbiome
Divy Mehra1, Thuy Doan2,3, Jason Betz1, Mireya Hernandez1, Jodi C Hwang1, Anat Galor1
1University of Miami Bascom Palmer Eye Institute, Miami, Florida; 2Proctor Foundation,
San Francisco, California; 3 Department of Ophthalmology, University of California, San Francisco, California
Purpose: Differences in composition of gut microbiota have been found in individuals with autoimmune diseases. There is data to suggest gut microbiome alterations, including bacterial diversity and constituency, in Sjögren’s Syndrome (SS), a common cause of immune-related dry eye (DE). This study evaluated microbial compositions of individuals with early or late markers of SS as compared to healthy controls.
Methods: Prospective study of 20 individuals with positive early markers (anti-SP1, anti-CA6, anti-PSP IgG, IgA, and IgM) or late markers of SS (anti-Ro/SS-A and anti-La/SS-B) with no other autoimmune diagnoses and 20 healthy controls, age- and sex-matched. Stool samples were collected for metagenomic RNA sequencing. The main outcome measure was gut microbial composition and diversity.
Results: 20 individuals with SS (mean age 60.6±12.9 years, Dry Eye Questionnaire-5 (DEQ-5) score of 14.8±3.6, Ocular Surface Disease Index (OSDI) score of 49.1±24.7, Schirmer of 7.6±5.2mm right eye and 8.2±5.7mm left eye) were compared to 20 healthy controls (mean age 58.8±11.7 years, DEQ-5 score of 4.5±4.0, OSDI score of 14.3±12.5, Schirmer of 22.2±9.3mm right eye and 21.9±9.1mm left eye). No statistically significant differences were observed in bacterial diversity between groups. Regarding genus composition, the relative abundance of Sneathia, Parascardovia, Pyramidobacter, and Veillonella were higher in the SS group compared to the control group. In addition, several species of Lactobacilli (L. salivarius, L. gasseri, L. reuteri, and L. johnsonii) were found at elevated levels in the SS group.
Conclusions: Differences in intestinal microbiome composition were found in individuals with early or late markers of primary Sjögren’s Syndrome as compared to an age and gender matched control group. Further studies are needed to elucidate the role of gut microbiota in Sjögren’s-related immune dysregulation.
Disclosure: N
Support: Supported by the Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Clinical Sciences R&D (CSRD) I01 CX002015 (Dr. Galor) and Biomedical Laboratory R&D (BLRD) Service I01 BX004893 (Dr. Galor), Department of Defense Gulf War Illness Research Program (GWIRP) W81XWH-20-1-0579 (Dr. Galor) and Vision Research Program (VRP) W81XWH2010820 (Dr. Galor), National Eye Institute R01EY026174 (Dr. Galor) and R61EY032468 (Dr. Galor), NIH Center Core Grant P30EY014801 (institutional) and Research to Prevent Blindness Unrestricted Grant (institutional).
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